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Fig. 1 | BMC Immunology

Fig. 1

From: Ionizing radiation modulates the phenotype and function of human CD4+ induced regulatory T cells

Fig. 1

TREGS are more radio-resistant than conventional CD4+ T cells. a Purified CD4+ TCONV cells or nTREGS were exposed to 10 Gy of radiation or mock irradiated (0 Gy). After 48 h, cells were stained with fixable viability stain (FVS) and CD4 or FVS, CD4, CD25, and Foxp3 to denote CD4+ TCONV and TREG cells, respectively. Representative plots and mean frequency (b) of each cell type are shown. c TGF-β1-induced TREGS or CD4+ TCONV cells were treated with 10 Gy of radiation or mock irradiated (0 Gy). 48 h post-treatment, cells were stained and analyzed by flow cytometry. Representative plots and mean frequency (d) of each cell type are shown. Experiment was repeated three times with similar results. Error bars represent SEM. *P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001 by paired, one-tailed Student t test

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