Fig. 4
From: Ionizing radiation modulates the phenotype and function of human CD4+ induced regulatory T cells
Phenotypic modulation of CD4 + CD25hi iTREGS by radiation. a CD4+ TCONV cells or b iTREG cells were mock irradiated or exposed to 10 Gy of radiation. Forty-eight hours post treatment CD4+ cells were analyzed for expression of CD25 by flow cytometry. c Representative plots of CD4 + CD25+ total (quadrant) or CD4 + CD25hi cells (box with numbers inset in plot). The expression of (d) CTLA-4, (e) LAG-3, (f) CD39, (g) CD73, (h) PD-L1, and (i) PD-L1 mean fluorescence intensity (MFI) were evaluated within the CD4 + CD25hi population 48 h after radiation. Experiment was repeated three times with similar results. Error bars represent SEM. *P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001 by paired, two-tailed Student t test