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Fig. 6 | BMC Immunology

Fig. 6

From: Ionizing radiation modulates the phenotype and function of human CD4+ induced regulatory T cells

Fig. 6

Irradiated iTREGS exhibit reduced suppressive capacity. CD8+ T cells were labeled with CFSE and stimulated to proliferate with CD3 and CD28. Irradiated or non-irradiated iTREGS were co-incubated with CD8+ T cells at a ratio of 1:4 for 5 days as described in Methods. a Histogram overlay displaying CD8+ T cell CFSE dilution alone (no added TREGS), in the presence of non-irradiated TREGS (middle portion of plot), or irradiated iTREGS (bottom portion of plot). b Percent of proliferating CD8+ T cells cultured alone, with mock irradiated TREGS (0 Gy), or 10 Gy treated iTREGS. Experiment was repeated three times with similar results. Error bars represent SEM. *P ≤ 0.05 by one-way ANOVA with Tukey’s post-hoc analysis for multiple comparisons

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