Fig. 4

Fs vaccines reduce spontaneous lung metastasis. a Quantification of pulmonary metastasis after immunization with Fs vaccines alone or (b) in combination with checkpoint blockade. At the endpoint, mice were euthanized, the lung aseptically removed, enzymatically treated and plated for 14 days. Fixed clonogenic 4 T1 metastatic colonies were stained with methylene blue and then counted. Bar graphs represent mean ± S.E and dots represent counts for each mouse evaluated. * p < 0.01; **, p < 0.001, analyzed by unpaired t test with Welch’s correction. Circle symbols in both vaccines represent re-challenged mice. Correlation between tumor weight (at endpoint) and number of pulmonary metastasis without (c) and with ICI treatment (d). Dots represent each mouse evaluated