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Fig. 5 | BMC Immunology

Fig. 5

From: Comparison of personal and shared frameshift neoantigen vaccines in a mouse mammary cancer model

Fig. 5

FS vaccination induces robust T-cell responses. IFN-γ positive splenocytes against shared BC-FAST antigens without (a) and with ICI (b). At the endpoint, mice splenocytes were harvested, stimulated in triplicate with pooled vaccine peptides (10 mg/ml per well of each peptide) (3–4 Fs antigen/well) and assessed by IFN-γ ELISPOT. Mouse splenocytes from the mock group were pooled and evaluated against all the peptide pools for BC-FAST. And against each of the PCV formulations (all vaccine antigens together) without (c) and with ICI (d). Bars represent mean ± S.E for the vaccine groups and dots represent per mouse immune response to the vaccine formulation (sum of the vaccine peptide pools). The Mock group was tested twice, and bars represent the mean ± S.E between replicates. (Circles) Re-challenged mice. * p < 0.05, analyzed by unpaired t test with Welch’s correction. Mock* = tested against BC-FAST vaccine peptides; MOCK** = tested against PCVs peptides

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