Fig. 2

Splenic lymphocyte function and Tms and Tregs proportions in skin-primed recipients on day 4 after the secondary heart transplant. a CD4⁺ Tm/CD4⁺ T cell proportions (top), CD8⁺ Tm/CD8⁺ T cell proportions (bottom); b Flow cytometry analysis of Tregs; Cumulative data analysis for the proportion of c CD4⁺ Tm/CD4⁺, d CD8⁺ Tm/CD8+ T cells and e Tregs (n = 3 mice/group); f Proliferation of recipient splenic T cells in response to donor BALB/c cell as assessed using MLR assay. Among the groups, the mean OD values were compared (n = 3 mice/group). Splenic T cells alone served as the negative control. Data are representative of three separate experiments (*, P < 0.05; **, P < 0.01; ***, P < 0.001 versus skin-primed HTm ctrl)