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Fig. 1 | BMC Immunology

Fig. 1

From: Human CD8+CD28− T suppressor cells expanded by common gamma chain (γc) cytokines retain steady allospecific suppressive capacity in vivo

Fig. 1

In vitro-expanded CD8+CD28− T cells possess durable suppressive activity at different time points in vitro. The in vitro-expanded CD8+CD28− T cells (2.5 × 104/well), which had been primed with B-APCs during the expansion culture, were tested for the ability to suppress proliferation of autologous CFSE-labeled CD4+ T cells (5 × 104/well) stimulated with allogenic APCs (5 × 104/well) in vitro for 7 days and 11 days. The top and bottom displayed in the histogram were the data expressed as percentage of CD4+ T cell proliferation in the B-APCs (top) or the I-APCs (bottom) stimulating group measured by CFSE dilution from a representative experiment after 7 days (a) or 11 days (c) of coculture. Statistical analysis displayed in bar graphs showed the suppression percentage of CD4+ T cells proliferation by the in vitro-expanded CD8+CD28− T cells in the B-APCs or the I-APCs stimulating group after 7 days (b) or 11 days (d) of coculture (n = 3). *** P<0.001

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BMC Immunology

ISSN: 1471-2172

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