Fig. 3From: Human CD8+CD28− T suppressor cells expanded by common gamma chain (γc) cytokines retain steady allospecific suppressive capacity in vivoCD8+CD28− T cells are induced by multiple mechanisms triggered by the common gamma chain cytokines. Some possible mechanisms accounting for the expansion of CD8+CD28− T cells by the common gamma chain cytokines IL-2, IL-7, and IL-15 were elucidated. a Proliferation. The proliferation of purified CD8+CD28− and CD8+CD28− T cells were measured in the presence or absence of the combination of common gamma chain cytokines. b Transformation. The percentage of CD8+CD28− T cells was shown when purified CD8+CD28+ T cells were cocultured in the presence or absence of the cytokines for 9 days. Isotype control antibody (grey) was used in the flow cytometry. c Survival. After 9 days of culture, the percentage of necrotic cells (Annexin V+ 7-AAD+) in the CD8+CD28+ and CD8+CD28− T cells was assessed by flow cytometry. Aggregated data from three experiments using different stimulator-responder pairs are shown (n = 3). *** P<0.001,* P<0.05Back to article page