Fig. 3
From: Progranulin inhibits LPS-induced macrophage M1 polarization via NF-кB and MAPK pathways
Inhibitory effects of rPGRN on CD86/CD206 ratio and inflammatory cytokines (TNF-α and iNOS) expression in LPS-stimulate RAW264.7 cells. Cells were treated with LPS plus 5, 10, 20, 40, and 80 ng/ml rPGRN for 24 h and normal medium containing equal amount of PBS to rPGRN and LPS groups was used as negative control. a, b CD86/CD206 ratio was detected by flow cytometry. c, d TNF-α (c) and iNOS (d) mRNA expression were determined with q-PCR. e-g TNF-α (e, f) and iNOS (e, g) protein expression were revealed by Western blot. h TNF-α secretion expression were evaluated by ELISA. N = <3, P < 0.05 (*), P < 0.01 (**), P < 0.001 (***) or p < 0.0001(****)