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Fig. 2 | BMC Immunology

Fig. 2

From: Involvement of ABC-transporters and acyltransferase 1 in intracellular cholesterol-mediated autophagy in bovine alveolar macrophages in response to the Bacillus Calmette-Guerin (BCG) infection

Fig. 2

The BCG infection altered intracellular levels of cholesterol and cholesterol ester in macrophages. Bovine AMs and murine macrophage RAW264.7 cells were infected with BCG at a dose of 10 for indicated times before their intracellular levels of cholesterol and cholesterol ester were ascertained. a-b Intracellular levels of cholesterol (a) and cholesterol ester (b) of primary bovine AMs altered by the infection of BCG. a The content of intracellular free cholesterol was increased gradually and reached its peak at 6 h post infection before it significantly decreased from 12 h afterward; b while the intracellular cholesterol ester content in AMs was significantly decreased and reached the lowest level at 6 h and then dramatically increased at 12 and afterward. c-d Intracellular levels of cholesterol (c) and cholesterol ester (d) of murine RAW264.7 cells altered by BCG. c The intracellular free cholesterol was increased and reached its peak at 12 h but reduced at 24 h post infection. A significant change of intracellular cholesterol was observed between 2 h, 6 h and 12 h after infection as compared with the control group; in contrast, d the intracellular cholesterol ester was significantly decreased and reached the lowest level at 12 h but increased at 24 h post infection. A significant change of intracellular cholesterol ester was determined between cells at 6 h, 12 h and 24 h following the infection, as compared with the control group. Data were expressed as mean ± SEM from three independent experiments. Compared to non-infection control, *: p < 0.05, **: p < 0.01

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