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Fig. 3 | BMC Immunology

Fig. 3

From: Involvement of ABC-transporters and acyltransferase 1 in intracellular cholesterol-mediated autophagy in bovine alveolar macrophages in response to the Bacillus Calmette-Guerin (BCG) infection

Fig. 3

BCG infection induced the expression of autophagy-related proteins in macrophages. Bovine AMs and murine macrophage RAW264.7 cells were infected with BCG at a dose of 10 for indicated times, and the expression of autophagy-related proteins LC3I/II and Beclin1 was determined by an immunoblotting (IB) assay. a Representative images of blots for LC3I/II and Beclin1 in BCG-infected primary bovine AMs (left panel), and the relative expression of proteins semi-quantified by a densitometry analysis (right panel) demonstrated an increased expression of autophagy-related proteins. b Representative images of blots of LC3I/II and Beclin1 in BCG-infected murine macrophage RAW264.7 cells (left panel), and their relative expression semi-quantified by a densitometry analysis (right panel) indicated an induced autophagy by BCG infection. Data were expressed as mean ± SEM from three independent experiments. Compared to non-infection controls, *: p < 0.05; **: p < 0.01

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