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Fig. 4 | BMC Immunology

Fig. 4

From: Involvement of ABC-transporters and acyltransferase 1 in intracellular cholesterol-mediated autophagy in bovine alveolar macrophages in response to the Bacillus Calmette-Guerin (BCG) infection

Fig. 4

An ACAT1-mediated alteration of intracelluar cholesterol and cholesterol ester in macrophages in response to BCG infection. Murine RAW264.7 cell lines overexpressing and silencing ACAT1 were generated by lentiviral infections with vectors overexpressing ACAT1 or shRNA to ACAT1, respectively. Control RAW264.7 cell lines infected with appropriate control lentiviral vectors were also generated. The transgenic RAW264.7 cells were infected with BCG at a dose of 10 for 12 h prior to being harvested for assessment of intracellular cholesterol (a) and cholesterol ester (b). a An overexpression of ACAT1 exhibited an ability to significantly suppress BCG-induced intracellular cholesterol, while knockdown of ACAT1 showed an enhanced level of intracellular cholesterol in RAW264.7 cells. b An overexpression of ACAT1 significantly restored BCG-repressed intracellular cholesterol ester, while knockdown of ACAT1 further reduced the BCG-repressed cholesterol ester in RAW264.7 cells. Data were expressed as mean ± SEM from three independent experiments. Compared to non-BCG-infected cells, *: p < 0.05; **: p < 0.01; compared to non-control lentivirus-infected cells, ∆: p < 0.05; ∆∆: p < 0.01

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