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Fig. 6 | BMC Immunology

Fig. 6

From: Involvement of ABC-transporters and acyltransferase 1 in intracellular cholesterol-mediated autophagy in bovine alveolar macrophages in response to the Bacillus Calmette-Guerin (BCG) infection

Fig. 6

The impact of ACAT1 signaling on the BCG-induced autophagy in RAW264.7 cells. The primary bovine AMs and RAW264.7 cells were infected with BCG at a dose of 10 in the presence of ACAT1 signaling inhibitor K604 for 12 h before the autophagy was determined by accessing autophagy-related proteins. a Representative blots demonstrated an increased expression of BCG-induced autophagy-related proteins ATG7, ATG5, LC3 and Beclin1 in primary bovine AMs in the presence of K604 (top panel), and the relative expression semi-quantified by a densitometry analysis (bottom panel) demonstrated an significant increase of autophagy-related proteins in comparison with cells without K604. b Representative blots demonstrated an increased expression of BCG-induced autophagy-related proteins ATG7, ATG5 LC3II/I and Beclin1 in RAW264.7 cells in the presence of K604 (top panel), and the relative expression semi-quantified by a densitometry analysis (bottom panel) demonstrated an significant increase of autophagy-related proteins in comparison with cells without K604. Data were expressed as mean ± SEM from three independent experiments. Compared to non-BCG-infected cells, *: p < 0.05; **: p < 0.01; compared to cells in the absence of K604, ∆: p < 0.05; ∆∆: p < 0.01

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BMC Immunology

ISSN: 1471-2172

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