Fig. 5
From: Phosphate starvation enhances phagocytosis of Mycobacterium bovis/BCG by macrophages
Cell membrane located PstS-1, LpqH, LprG, and APA are expressed by Pi-deprived mycobacteria. M.bovis/BCG bacilli were cultured with (+) and without Pi (−) for 24 h and the membranes were isolated by sonication and centrifugation, separated by 12% SDS-PAGE and stained with Coomasie blue (a). The membranes were transferred to PVDF for immunoblot with an anti-M.bovis/BCG antiserum which detected many protein bands more numerous in membranes of Pi-deprived bacilli (b). Using Abs available in our laboratory the expression of PstS-1, LpqH, LprG, and APA was demonstrated in isolated plasma membranes of Pi-deprived mycobacteria (c-f)