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Fig. 3 | BMC Immunology

Fig. 3

From: JAK inhibitors impair GM-CSF-mediated signaling in innate immune cells

Fig. 3

Effects of JAKi on JAK2 phosphorylation in GM-CSF stimulated THP-1 cells. a THP-1 cells were pretreated with JAKi (tofacitinib, baricitinib, upadacitinib) at the indicated concentrations (400 nM) for 1 h and then stimulated with GM-CSF (20 ng/ml) for 20 min. Phosphorylation of JAK2 was determined by Western blotting using phospho-specific antibodies against JAK2. b THP-1 cells were pretreated with JAKi (tofacitinib, baricitinib, upadacitinib) at the indicated concentrations (25, 100 nM) for 1 h and then stimulated with GM-CSF (20 ng/ml) for 20 min. Phosphorylation of JAK2 was determined by Western blotting using phospho-specific antibodies against JAK2. Phosphorylation levels of JAK2 were normalized to total protein levels. Mean ± SD of the ratio of phosphorylated JAK2 to total JAK2 of three independent experiments was shown. Statistical significance between GM-CSF-stimulated cells and each JAKi-pretreated cells was determined. *p < 0.05

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