Fig. 6
From: JAK inhibitors impair GM-CSF-mediated signaling in innate immune cells
Effects of JAKi on pro-IL-1β mRNA expressions in GM-CSF-stimulated neutrophils. Neutrophils (1 × 106/ml) were stimulated with GM-CSF (20 ng/ml) for 8 h in the presence or absence of the pretreatment JAKi (tofacitinib, baricitinib, Upadacitinib) for 1 h. The cells were harvested and analyzed for pro-IL-1β and GAPDH mRNA levels by real-time PCR. Values represent the mean ± SD of two independent experiments