Fig. 7
From: JAK inhibitors impair GM-CSF-mediated signaling in innate immune cells
NLRP3 expression in neutrophils. Neutrophils were stimulated with GM-CSF for 24 h in the presence or absence of the pretreatment JAKi (tofacitinib, baricitinib, Upadacitinib) for 1 h. Cellular lysates were analyzed by Western using anti-NLRP3 or anti-β-actin antibodies. NLRP3 protein expression were normalized to the protein expression of β-actin. Mean ± SD of the ratio of NLRP3 to β-actin of three independent experiments was shown. Statistical significance between GM-CSF-stimulated cells and each JAKi-pretreated cells was determined. *p < 0.05