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Fig. 2 | BMC Immunology

Fig. 2

From: EGR2 is elevated and positively regulates inflammatory IFNγ production in lupus CD4+ T cells

Fig. 2

Increased percentage of EGR2 expressing cells and EGR2 expression intensity in splenic CD4+ T cells of MRL-lpr and B6.sle123 mice. The freshly-isolated splenocytes were stained with cell surface marker CD4, and then subjected to intracellular flow stain of EGR2. (a-c) Intracellular flow cytometry analysis of EGR2 expression in gated splenic CD4+ T cells of MRL and MRL-lpr at 5 weeks-of age and15 weeks-of age. The representative histogram plot shows EGR2 protein expression in gated CD4+ T cells of MRL-lpr and control MRL mice at different age (a). The summary graphs show the percentage of EGR2 expressing cells in CD4+ T cells (EGR2+/CD4+, b) and EGR2 expression intensity (MFI, c) in MRL-lpr and MRL CD4+ T cells. Graphs show means ± SEM (n ≥ 4). (d-f) Intracellular flow cytometry analysis of EGR2 protein expression in gated splenic CD4+ T cells of B6 and B6.sle123 lupus mice. The representative histogram plot shows EGR2 protein expression in gated splenic CD4+ T cells of B6.sle123 (26–27-week- old) and age-matched control B6 mice. The summary graphs show the percentage of EGR2-expressing cells (e) and EGR2 MFI (f) in B6.sle123 and control B6 CD4+ T cells. Graphs show means ± SEM (n = 3 each). One-way ANOVA with Tukey- Kramer all pair’s comparisons were performed for statistical analysis of multiple groups (b and c). The groups that were not connected with the same letter were significantly different in their means. Unpaired student t-tests (B6 vs B6.sle123); *, p < 0.05, and **, p < 0.01

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