Fig. 6
From: EGR2 is elevated and positively regulates inflammatory IFNγ production in lupus CD4+ T cells
T cell activation induces EGR2 expression in different lymphocyte subsets of both B6 and B6.sle123 mice. The freshly-prepared (t0), anti-CD3and anti-CD28 stimulated (24 h of stimulation) splenocytes from B6.sle123 and control B6 mice (31–32-week-old) were stained with cell surface markers CD4, CD8, B220, and then subjected to intracellular flow stain of EGR2. The cells were gated on CD4+ T, CD8+ T, and B220+ B cells respectively to analyze the expression of EGR2. The graphs show the percentage of EGR2 expressing cells and EGR2 expression intensity (MFI) in CD4+ T (a & b), CD8+ T (c & d), B220+ B (e & f) cells. Graphs show means ± SEM (n ≥ 4). One-way ANOVA with Tukey- Kramer all pair’s comparisons were performed for statistical analysis. The groups that were not connected with the same letter were significantly different in their means