Fig. 3

CITRUS analyses for the stimulated cells, clustering on all 28 surface and intracellular markers and comparing cell abundance in the three groups. a CITRUS tree in which each node denotes different cell clusters. The red nodes illustrate cell populations were the cell abundance differed statistically significantly between the three groups as determined by SAM analyses, FDR 0.05. b Cell abundance as proportion of all cells, shown as box plots (expressing IQR and median values) and values for each individual within the groups of healthy controls, IgEpos, and IgEneg food allergic subjects, for the clusters identified in A. Differences between groups are shown as lines, * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001 (Kruskal-Wallis and Dunn’s multiple comparisons test). c CITRUS histograms for the clusters identified in A. For each surface marker, the histogram shows the marker expression on cells in the specific cluster (red) against the marker expression on all other cells (blue) – overlapping of histograms will result in purple areas