Table 3 Immune populations assessed by biaxial gating, and the markers used for their identification; parent populations are indicated italic

Panel 1

T cells

CD3+

γδ Tcells

CD3+

TCRgd+

Natural killer T cells (NKT)

CD3+

CD56+

T double positive

CD3+

CD8+

CD4+

T double negative

CD3+

CD8-

CD4-

T cytotoxic (Tc)

CD3+

CD8+

Tc central memory

CD3+

CD8+

CD45RA-

CCR7+

Tc effector

CD3+

CD8+

CD45RA+

CCR7-

Tc effector memory

CD3+

CD8+

CD45RA-

CCR7-

Tc HLA-DR+

CD3+

CD8+

HLA-DR+

Tc naive

CD3+

CD8+

CD45RA+

CCR7+

T helper (Th)

CD3+

CD4+

Th 2

CD3+

CD4+

CCR4+

CRTH2+/CD294

Th central memory

CD3+

CD4+

CD45RA-

CCR7+

Th effector

CD3+

CD4+

CD45RA+

CCR7-

Th effector memory

CD3+

CD4+

CD45RA-

CCR7-

Th follicular (Th FC)

CD3+

CD4+

CD4+

CD185+

Th activated HLA-DR+

CD3+

CD4+

HLA-DR+

Th naive

CD3+

CD4+

CD45RA+

CCR7+

Th 1

CD3+

CD4+

CXCR3+

Th 17

CD3+

CD4+

CXCR3-

CCR6+

T regulatory (Tregs)

CD3+

CD4+

CD25+

CD127-

B cells HLA-DR+

CD19+

HLA-DR+

B cells double negative

CD19+

HLA-DR+

IgD-

CD27-

B cells naive

CD19+

HLA-DR+

IgD+

CD27-

B cells non-class-sw. mem.

CD19+

HLA-DR+

IgD+

CD27+

B cells class-sw. mem.

CD19+

HLA-DR+

IgD-

CD27+

Plasmablasts

CD19+

HLA-DR+

IgD-

CD27+

CD38++

Monocytes (Mo)

CD3-

CD19-

CD56-

CD45+

CD33+

CD14+/++

CD16-/+/++

Mo classical

CD3-

CD19-

CD56-

CD45+

CD33+

CD14++

CD16-

Mo intermediate

CD3-

CD19-

CD56-

CD45+

CD33+

CD14++

CD16+

Mo non-classical

CD3-

CD19-

CD56-

CD45+

CD33+

CD14+

CD16++

Dendritic cells plasmacytoid

CD3-

CD19-

CD56-

CD45+

CD14-

HLA-DR+

CD123+

Dendritic cells myeloid (mDC)

CD3-

CD19-

CD56-

CD45+

CD14-

HLA-DR+

CD11c+

Natural killer cells (NK)

CD3-

CD19-

CD14-

CD33-/+

CD161+

CD56+

NK precursor

CD3-

CD19-

CD14-

CD33-/+

CD161+

CD56-

NK mature

CD3-

CD19-

CD14-

CD33-/+

CD161+

CD56+

CD45

CD56dim

NK immature

CD3-

CD19-

CD14-

CD33-/+

CD161+

CD56+

CD45

CD56bright

ILC2s

CD3-

CD19-

CD16-

CD56-

CD14-

CD161-

CD127+

CRTH2+/CD294+