Fig. 4

B-cell activation in low-dose immunization protocol occurs in the withers adipose tissue, but not in the lymph nodes. a: Expression of B-cell specific gene cd19 and the genes associated with B-cell antibody class switching Aicda, germline ε and γ1 in the withers (W) or axillary lymph nodes (LN) after 14th s.c.w. with saline (0), 100 or 10,000 ng/injection of OVA. All data were normalized to intact control. b: expression of genes linked with immune cell activation bcl6, ebi2, gata3, and nmur1. c: expression of innate-immunity linked pro-allergic cytokines genes IL-25, 33, and TSLP in the same samples. Data are representative from 3 independent experiments (n = 5 mice per group, total number of mice 15). Error bars show SD. Statistical significance between immunized and intact mouse groups is shown with asterisks: * - p < 0.05; ** - p < 0.01; *** - p < 0.001; statistical significance between low and high dose groups is shown in each figure in upper right pannel