Fig. 6
Water soluble and lipophilic anti-proliferative drugs stabilize and enhance specific IgE rather than IgG1 production. BALB/c mice were immunized by low (100 ng) dose of OVA alone or with low doses (2 and 20 μg) of anti-proliferative drugs Doxorubicin (D 2 and D 20) and Etoposide (E 2 and E 20) in withers tissue. Three days after the 7th immunization (2 weeks after the start, 2 w) and 3 days after the last immunization (4 weeks after the start, 4 w) blood was taken for the measurement of antigen-specific humoral response. a: Specific IgE antibody production. b: Specific IgG1 antibody production. Antibody production was compared between saline and immune mice as well as between control (antigen alone immunized) mice and mice immunized together with anti-proliferative drugs. Total number of mice 23, 6 mice in saline and 7 in antigen control group and 5 mice in each test group. Mann-Whitney test was used for p value estimation.* - p < 0.05; ** - p < 0.01 from p.i. and saline immunized mice, § - p < 0.05 between bar indicated groups