Fig. 4
From: Association of TIM-3 expression with glucose metabolism in Jurkat T cells
Mitochondrial DNA content, membrane potential, and ROS production in TIM-3 overexpressing or knockout cells. Mitochondrial DNA content (a), membrane potential (b), and ROS production (c), in control cells (JLV cell line, G2 clone, and CON clone), TIM-3 overexpressing cells (JLT3 cell line and T7 clone), and TIM-3 knockout cells (TIM3KO clone) cultured in the absence (−) or presence (+) of PMA (25 ng/ml) and Iono (10 μM) for 6 h. Mitochondrial DNA content was measured using qPCR. Mitochondrial membrane potential and ROS production were assessed by using flow cytometry with DilC(5) and MitoSOX™, respectively. Data represent two independent experiments performed in triplicates or sextuplets (JLT3 cell line and T7 clone). Data represent an experiment performed in sextuplets (TIM3KO clone). Data are mean ± SD. Mitochondrial DNA ratio: mitochondrial DNA to the nuclear 18S DNA. P/I: PMA and Iono. *: P < 0.05