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Fig. 6 | BMC Immunology

Fig. 6

From: Association of TIM-3 expression with glucose metabolism in Jurkat T cells

Fig. 6

Transcript levels of Glut1, 2, 3 and 4 in TIM-3 overexpressing or knockout cells. Transcript levels of Glut2, 3, and 4 relative to Glut1 in each cell line in the absence of stimulation (a). mRNA levels of Glut1 (b), Glut2 (c), Glut3 (d), and Glut4 (e) in control cells (JLV, G2, and CON), TIM-3 overexpressing cells (JLT3 and T7), and TIM-3 knockout cells (TIM3KO) stimulated with PMA (25 ng/ml) and Iono (10 μM) for the indicated time was determined using qRT-PCR. Relative transcript: mRNA level of each cell line at the indicated time point relative to mRNA level in the corresponding control cells at the 0 time point. Data represent two independent experiments performed in triplicate or sextuplicate. Data are mean ± SD. *: P < 0.05

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