Fig. 7
From: Association of TIM-3 expression with glucose metabolism in Jurkat T cells
Glut1 protein level in TIM-3 overexpressing or knockout cells. Glut1 protein levels analyzed by using flow cytometry in TIM-3 overexpressing cells (a and b), and TIM-3 knockout cells (TIM3KO) (c and d) along with the corresponding control cells (JLV, G2, and CON, respectively). Cells stimulated for the indicated time with PMA (25 ng/ml) and Iono (10 μM) were labeled with PE-conjugated anti-mouse Ig Ab alone or together with mouse anti-Glut1 Ab and then the mean fluorescence intensity (MFI) was assessed. Representative histogram (c). Data represent two independent experiments performed in triplicate or sextuplicate. Data are mean ± SD. Fold of MFI: MFI relative to MFI of corresponding control cells at the 0 time point. P/I: PMA and Iono. *: P < 0.005