Fig. 2
Mice housed under SPF/barrier conditions lack differentiated memory CD8+ T cell subsets compared with feral mice, pet store mice and adult humans. a Percentages of CD8+ T-cell phenotypes in laboratory/SPF mouse blood, adult human blood and human neonatal cord blood. The top panels are gated on CD3+/CD8+ cells with naïve T cells highlighted in blue, central memory T cells highlighted in green and effector memory T cells highlighted in red as defined by established lineage markers in each species. Bottom panels are gated on antigen-experienced subsets as defined in the green and red quadrants above). b Percentages of granzyme B+/CD8+ T-cells are much less in the antigen-experienced subsets of laboratory mice and neonatal humans when compared with adult humans. c and d Percentages of naïve CD8+ T cells (CD44lo/CD62Lhi) in PBMCs from laboratory mice are much greater than those in PBMCs from feral and pet store mice whereas percentages of antigen-experienced C8+ T cells (CD44hi/CD62Llo) were significantly greater in feral and pet store mice when compared those in laboratory mice. Reproduced from [12] with permission