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Fig. 2 | BMC Immunology

Fig. 2

From: Small RNA sequencing of extracellular vesicles identifies circulating miRNAs related to inflammation and oxidative stress in HIV patients

Fig. 2

Characterization of EV fractions isolated from plasma. a. TEM image of isolated EV fraction from a HIV-negative control subject. b. Size distribution of EVs in a representative HIV-negative and HIV-positive subject by nanoparticle tracking analysis (NTA). c. Detection of exosome markers CD9, Flotillin-1, Tsg101, and CD81 by western blotting in pooled HIV-positive (n = 3) and HIV-negative (n = 3) samples. Three individual HIV-negative subjects were matched for age, gender, race, and cocaine use to three HIV-positive subjects to achieve matching between the pooled samples. Equal amounts of protein (50 μg per lane) were loaded for western blotting; these samples contain exosomal proteins, as well as co-purifying non-exosomal proteins from other sources such as microparticles and residual plasma proteins. Full-length blot images are shown in Supplementary Figure S6. d. EV concentration (left) and median EV size (right) analyzed by NTA in HIV-negative and HIV-positive subjects

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BMC Immunology

ISSN: 1471-2172

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