Fig. 2
Automated microscopy based assay for ADE works comparable to flow cytometry. K562 cells were infected with ZIKV, WNV or DENV-1 RVPs in the presence of indicated dilutions of WNV immune sera from a symptomatic WNV+ subject. Samples were analyzed either by automated microscopy or via flow cytometry. a Representative microscopy images of whole wells of a 96 well plate infected with ZIKV RVPs in the presence of indicated dilutions of WNV immune sera. b Read out of enhancement of respective RVP infection by automated microscopy. c Read out of enhancement of respective RVP infection after flow cytometry analysis. d Correlation analysis of measurement of GFP+ cells by flow cytometry versus automated microscopy (R2 = 0.9439)