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Fig. 3 | BMC Immunology

Fig. 3

From: Enhancement of Zika virus infection by antibodies from West Nile virus seropositive individuals with no history of clinical infection

Fig. 3

RVP based assay can differentiate between DENV, WNV and ZIKV sera in both ADE and neutralization assays. Vero cells were infected with (a) WNV (b) ZIKV or (c) DENV GFP RVPs in the presence of media alone or serial dilutions of WNV+, ZIKV+ or DENV+ sera samples. WNV+ sera was from a symptomatic WNV exposed subject as confirmed by WNV E IgG ELISA, and ZIKV and DENV+ sera were from subjects positive for ZIKV or DENV antibodies confirmed by specific NS1 IgG ELISA. The number of GFP positive cells was analyzed 72 h post infection. All wells were assayed in duplicates and graphs were plotted after normalizing data to 100% infection in the absence of any sera. K562 cells were infected with (d) WNV (e) ZIKV or (f) DENV GFP RVPs as in parts A-C above. The number of GFP positive cells was analyzed 48 h post infection. All wells were assayed in duplicates and graphs were plotted after normalizing data to peak ADE as 100% infection

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BMC Immunology

ISSN: 1471-2172

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