Fig. 6

Sera from ZIKV seropositive donors effectively neutralizes ZIKV infection. Vero cells were infected with (a) ZIKV (b) WNV or (c) DENV GFP RVPs in the presence of media or serial dilutions of a WNV+ serum sample (symptomatic) or ZIKV+ serum samples obtained from BEI resources. The number of GFP positive cells was determined 72 h post infection. All wells were assayed in duplicates and percent infection determined after normalizing data to 100% infection in the absence of any sera. K562 cells were infected with (d) ZIKV (e) WNV or (F) DENV GFP RVPs in the presence of serial dilutions of a WNV+ serum sample (symptomatic) or ZIKV+ sera samples. The number of GFP positive cells was analyzed 48 h post infection and normalized to peak ADE as 100% infection. g Serum dilution that causes 50% neutralization of ZIKV, WNV and DENV RVPs was determined for each sample. h Serum dilution that causes peak ADE for WNV, ZIKV and DENV RVPs was determined