Fig. 1

M1 and M2 polarized BMDM respond to C1q with an enhancement of phagocytosis. BMDM were either nonpolarized or polarized overnight to an M1 phenotype (classically activated) or an M2 phenotype (alternatively activated) and then adhered to 4 μg/mL HSA (control, white bars) or C1q (grey bars) for 30 min. BMDM were then incubated with EAIgG for an additional 30 min. Scoring was performed blinded with at least 200 cells per well scored. The phagocytic index was calculated as the total number of red blood cells in 100 macrophages, multiplied by 100. Bars represent the average of two individual wells from three separate experiments ± standard error of the mean. Each point represents a replicate. ** p ≤ 0.01, paired student’s t-test