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Fig. 2 | BMC Immunology

Fig. 2

From: Extracellular signal-regulated kinase 1/2 is required for complement component C1q and fibronectin dependent enhancement of Fcγ- receptor mediated phagocytosis in mouse and human cells

Fig. 2

C1q-dependent enhancement of phagocytosis does not require LAIR-1. BMDM were obtained from wild-type and LAIR-1 knockout (LAIR-1−/−) mouse femurs. a Wildtype and LAIR-1−/− BMDM were adhered to 4 μg/mL HSA (control) or C1q, and the phagocytosis assay was performed as described in the legend for Fig. 1. Arrows point to phagocytic cells. b LAIR-1 was not detected by western blot in the LAIR-1 −/− macrophages (inset). Percent phagocytosis was determined by dividing the number of macrophages ingesting at least one target by the total number of macrophages counted, multiplied by 100. The phagocytic index was calculated as the total number of red blood cells in 100 macrophages, multiplied by 100. Scoring was performed blinded with at least 200 cells per well. Bars represent the average of eight individual wells from two separate experiments + standard error of the mean. * p ≤ 0.05, paired student’s t-test

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