Fig. 3

C1q regulates ERK1/2 phosphorylation in IC-stimulated BMDM and THP-1. a BMDM were adhered to 4 μg/mL HSA (control) or C1q for 30 min and then incubated with IC for an additional 0, 10 min, or 30 min. After the indicated time, cells were lysed and levels of phospho-proteins and control proteins were detected by western blot. One representative western blot from 3 to 6 replicates is shown b BMDM were processed as described in a and phosphorylated ERK1/2 (p-ERK) was normalized to either total ERK1/2 (n = 3) or actin (n = 3) loading controls at 0 and 10 min using ImageJ. Bars represent the mean of 6 individual experiments with SEM and points represent individual experiments. *p < 0.05 paired student’s t-test for time zero control versus C1q. c THP-1 cells were processed as described in a and one representative blot out of three is shown. All western blot images were cropped to display relevant bands. Corresponding uncropped full-length blots are included in supplementary Fig. 2