Fig. 4

PD0325901 inhibits C1q-dependent enhancement of phagocytosis of EAIgG. a BMDM were adhered to 4 μg/mL HSA (control) or C1q for 30 min then treated with the vehicle control (DMSO) or PD0325901 for an additional 15 min. BMDM were then incubated with EAIgG and processed for phagocytosis as described in the legend to Fig. 1. Bars represent the mean of 3 individual experiments + SEM. *p ≤ 0.05 using a paired Student’s t-test. b THP-1 cells were processed as described in a. Bars represent the mean of 3 individual experiments + SEM. *p ≤ 0.05 paired student’s t-test. n.s. = not significant. c BMDM were adhered to control protein or C1q as described in a then treated with the vehicle control (DMSO) or VX-745 for 15 min. BMDM were then processed for phagocytosis. Bars represent the mean of 3 individual experiments + SEM. d Raw264.7 cells were either untreated or pre-treated with 250 nM PD3259901 or 200 nM VX-745 for 15 min prior to the addition of 300 ng/ml LPS for an additional 4 h. Supernatants and cell lysates were harvested and TNFα measured by ELISA. Bars represent duplicate or triplicate wells (±SD) from one experiment representative of two