Fig. 5

C1q and fibronectin upregulate phagocytosis of EAIgGs through a similar ERK1/2 mechanism. a BMDM were adhered to 4 μg/mL HSA (control), C1q, or fibronectin (Fn) for 30 min and then incubated with BSA-anti-BSA immune complexes (IC) for an additional 10 or 30 min. After the indicated time, cells were lysed and levels of ERK1/2 phosphorylation and total ERK1/2 were detected by western blot and analyzed using ImageJ. Bars represent the average of 3 individual experiments + SEM. Representative western blot depicted (top) aligns with quantification sample order (bottom). b BMDM were adhered to 4 μg/mL HSA (control), C1q, or Fn for 30 min and treated with the vehicle control (DMSO) or PD0325901 for 15 min, incubated with EAIgG, and processed for phagocytosis as described in previous figure legends and materials and methods. Bars represent the mean of 3 individual experiments normalized + SEM. *p ≤ 0.05 using a Student’s t-test. All western blot images were cropped to display relevant bands. Corresponding uncropped full-length blots are included in supplementary Fig. 2