Fig. 6

C1q downregulates ERK1/2 phosphorylation and TNFα production. BMDM were adhered to 4 μg/mL HSA (control) or C1q for 30 min, treated or not treated with IC for an additional 15 min, and then incubated with 300 ng/mL LPS for 4 h. a Cells were lysed and levels of ERK1/2 phosphorylation and total ERK1/2 were detected by western blot and analyzed using ImageJ. Bars represent average p-ERK divided by total ERK from 3 individual experiments + SEM. Representative western blot depicted (top) aligns with quantification sample order (bottom). *p ≤ 0.05 paired student’s t-test. b BMDM supernatants were collected from samples described in a and TNFα was measured by ELISA. Bars represent the mean of 10 individual experiments **p ≤ 0.01 paired student’s t-test. All western blot images were cropped to display relevant bands. Corresponding uncropped full-length blots are included in supplementary Fig. 2