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Fig. 1 | BMC Immunology

Fig. 1

From: The role of β2 integrin in dendritic cell migration during infection

Fig. 1

Migration of macrophages in the colon post-infection is affected by the lack of functional β2 integrin. Itgb2mut and WT littermate controls were infected with 200 embryonated T. muris eggs and colon tissue were analysed by immunohistochemistry staining at day 0 (D0) and day 19 (D19) post-infection(p.i) to identify epithelial cells with cytokeratin (green), CD11c (red) (a, b) or F4/80 (c, d) expressing cells (red) and counterstained with DAPI (blue). Shown are representative images at D19 p.i. (a, b, c, d) from one of two experiments. The number of (e) CD11c+ cells and (f)  F480+ cells were enumerated by calculating the mean of 5 fields/per mouse, n = 3–8 mice per time point. CD11b+F4/80+ macrophages were analysed by flow cytometry, (g) Absolute numbers of CD11b+F480+ macrophages as a proportion of the CD45+CD11c+MHClo population were calculated, n = 4–5 mice per time point, *(P < 0.05), **(p < 0.01)

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