Fig. 2

Recruitment of dendritic cells in response to infection was impaired by the lack of functional β₂ integrin. Itgb2^mut and WT controls were infected with 200 embryonated T. muris eggs and CD11c⁺CD103⁺ DCs in the colon were assessed by immunohistochemistry using CD11c (red), CD103 (green) and counterstained with DAPI. Representative images of immunohistochemistry staining at day 0 (a, c), and D19 (b, d) post-infection in WT and Itgb2^mut mice from one of two experiments. (e) CD11c⁺CD103⁺ DCs in the colon were enumerated and the mean of 5 fields of view per mouse was plotted, n = 4–8 mice /time point. (f, g, h, i) Colonic DCs were isolated both from WT and Itgb2^mut mice at D0 and D19 post infection, data were analysed by flow cytometry to assess DC subsets in the colon. Absolute numbers of CD45⁺ cells were calculated for (f) CD11c⁺MHCII⁺F4/80⁻, (g) CD103⁺CD11b⁺, (h) CD103⁺CD11b⁻, and (i) CD103⁻CD11b⁺ (I) DC subsets in the colon, n = 4–5 mice/time point, *(P < 0.05), **(p < 0.01)