Fig. 6
From: The role of β2 integrin in dendritic cell migration during infection
Itgb2mut mice did not develop infection induced goblet cell hyperplasia in response to infection with T. muris and had fewer MUC-2 expressing cells in the colon than littermate controls in response to infection. Itgb2mut and WT littermate controls mice were infected with 200 embryonated T. muris eggs and harvested at Day 0 (D0) (a, b), D5, and D19 (c, d) post-infection. Representative images of colons taken from one of two experiments that were assessed by PAS staining for the quantification of goblet cells (a-d) or Muc2 to identify mucin-2 expressing cells (e-h). Images were analysed using Image J software and (i) the number of goblet cells, (j) the number of Muc-2 expressing cells, and (k) the ratio of goblet to epithelial cells were calculated as the mean of 20 measurements /tissue section, n = 4–8/time point. *(P < 0.05), (**p < 0.01)