Fig. 2

Flow cytometry anlysis of CD63-based BAT. a BAT was evaluated using the basophil activation marker CD63. At the time of subject enrollment, blood samples were collected, and the peripheral blood basophils were stimulated with an inhalant allergen mix. CCR3⁺ cells with low side scatter were considered basophils, and the CD63⁺ basophils were termed “activated basophils”. b The median levels of CD63 expression by basophils after inhalant allergen stimulation were 60.0% (34.8–84.2%) and 29.2% (11.1–77.4%) in children diagnosed with asthma and those not diagnosed with asthma, respectively. Data are expressed as the median (IQR); a Mann-Whitney rank sum test was used to compare the difference between groups (*, p < 0.05)