Fig. 2

Cellular composition of BALB/c spleens recovered after aerosol exposure to clinical strains of B. pseudomallei. Spleen cells were prepared as described in Material and Methods and labeled for CD4+ and CD8+ T cells, B cells, monocyte/macrophages, NK cells, and granulocytes for identified by flow cytometry. The data was from the analysis of a total of 75 spleens from 4 groups of spleens. A comparision of the composition of isolated spleens placed into different groups is shown: Gp 1, naïve; Gp 2, normal appearing; Gp 3, enlarged swollen; and Gp 4, chronically infected. The results are reported as the geometric mean of the percentage of cells with the standard error of the mean. The number in parenthesis after each group represents the number of spleens in each group, and each determination was completed at least once for each spleen. For spleens from naïve mice (n = 10), the cellular composition was the following: CD4⁺ T cells, 23.51% (1.03); CD8⁺ T cells, 9.96% (1.04); B cells, 50.7% (1.04); monocytes/macrophages, 4.38% (1.11); NK cells, 4.29% (1.12); and granulocytes, 0.89% (1.19)%. The significant levels compared to the cells from naïve mice (Gp 1) as shown: †, P < 0.05; ¶, P < 0.01; ‡, P ≤ 0.0001. The type and number of each strain (in parenthesis) in each group are as follows: Gp. 2, MSHR668 (5), 406e (3), K96243 (3), MSHR5855 (7), MSHR (5848 (6), HBPUB10134a (8); Gp. 3, 406e (1), 1106a (2), MSHR305 (1), MSHR668 (1), K96243 (1), 1026b (1), MSHR5854 (1); Gp. 4, MSHR668 (1), 406e (2), 1106a (6), MSHR305 (3), K96243 (2), 1026b (3), Bp22 (2), MSHR5858 (4), MSHR5855 (1), MSHR5848 (1)