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Fig. 4 | BMC Immunology

Fig. 4

From: Dysregulation of TNF-α and IFN-γ expression is a common host immune response in a chronically infected mouse model of melioidosis when comparing multiple human strains of Burkholderia pseudomallei

Fig. 4

Histochemical and immunohistochemical analysis of spleens from mice exposed by aerosol to B. pseudomallei shows the pyogranulomatous lesions and the presence of the pathogen. Figures are representative of 16 separate spleens from 6 studies containing 60 mice (5 naïve mice and 11 exposed mice). Results are shown for the following spleens: A. a spleen from a naïve BALB/c mouse, 10 weeks old; B. a normal appearing spleen from a BALB/c mouse exposed to 1 CFU of B. pseudomallei 22 after 22 days PI; C and D infected spleens from BALB/c mice exposed to 2851 CFU of B. pseudomallei 1106a after 49 days PI, one grossly infected and the other an early minor infected spleen, respectively. Column 1 shows the picture of the respective spleens before analysis. Column 2 shows paraffin sections of the respective spleens stained with hematoxylin and eosin (H&E) that shows the presence of pyogranulomatous lesions present in the infected spleens (B,C,D). Scale bar shown on lower right. Column 3 shows the presence and location of immunoreactive exopolysaccharide material of B. pseudomallei present in infected spleens at low magnification (IHC-Low) relative to the whole spleen. Scale bar shown on lower right. Column 4 shows a higher magnification (IHC-High) of the immunoreactive material on the edge of pyogranulomatous lesions with stained epithelial macrophages surrounding some lesions. Scale bar shown on lower right

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