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Fig. 5 | BMC Immunology

Fig. 5

From: Dysregulation of TNF-α and IFN-γ expression is a common host immune response in a chronically infected mouse model of melioidosis when comparing multiple human strains of Burkholderia pseudomallei

Fig. 5

Detection of inflammatory cytokine expression in B. pseudomallei exposed mouse spleens. A total of 12 spleens were each examined at least once with 4 different RNA hybridization probes: 2 spleens from naïve BALB/c mice, 5 normal appearing spleens from exposed BALB/c, and 5 infected spleens from BALB mice. Examples of results from a control naïve mouse and 2 infected spleens from 2 BALB/c are shown. In situ RNA hybridization was used to detect the expression of IL-1α (panel 1), IL-1β (panel 2), TNF-α (panel 3), and IFN-γ (panel 4) in the spleens. A. A spleen from a control naïve mouse is shown in A. B. A spleen from a BALB/c mouse exposed to 2851 CFU of B. pseudomallei 1106a 49 days PI with a massive, multifocal pyogranulomatous growth occupying more than half of the spleen. Many reactive cells can be seen for IL-1α, IL-1β, TNF-α RNA probes at the outer edge of a capsular structure containing multiple, unreactive pyogranulomatous nodules and in separate pyogranulomatous lesions. Much fewer scattered IFN-γ reactive cells could be seen in the same areas. C. A spleen from a BALB/c mouse exposed to 2851 CFU of B. pseudomallei 1106a 49 days PI, showing an early development of a multifocal pyogranulomatous lesion. Many reactive cells can be seen for IL-1α, IL-1β, and TNF-α in the outer edge of pyogranulomatous lesions. Many reactive cells can also be seen in the fibrotic epithelial macrophage region surrounding the pyogranulomatous lesions. Few IFN-γ reactive cells can be seen near the pyogranulomatous lesion

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