Fig. 6

Intracellular expression of TNF-α and IFN-γ in spleen cells from B. pseudomallei exposed BALB/c mice. Examples of spleen cells (grouped according to their characteristics, see Fig. 1) from a total of at least 49 B. pseudomallei (included 8 different strains) aerosol exposed BALB/c mice and from 10 naïve BALB/c mice were analyzed for the intracellular expression of TNF-α (A) and IFN-γ (B) are shown. Intracellular cytokine expression was detected with anti-IFN-γ-APC or anti-TNF-γ-APC after cells were surface labeled and permeabilized. The results are reported as the percentage of cell type (geometric means with SEM) containing TNF-α or IFN-γ, and analysis of each spleen was determined at least once. The percentage of cell type (geometric mean with SEM) in naïve BALB/c mice that expressed intracellular TNF-α/IFN-γ were as follows: CD4+ T cells, 0.17 (1.45)%/0.13 (.132)%; CD8+ T cells, 0.17 (1.41)%/0.06 (1.30)%; B cells, 0.48 (1.30)%/0.59 (1.11)%; monocyte/macrophages, 0.05 (1.25)%/0.12 (1.23)%; NK cells, 0.09 (1.87)%/0.09 (1.39)%; granulocytes, 0.01 (1.74)%/0.06 (1.18)%. The type and number of each strain (in parenthesis) in each group are as follows: Gp. 2, MSHR668 (5), 406e (3), K96243 (3), MSHR5855 (7), MSHR5848 (6), HBPUB10134a (4); Gp. 3, 406e (1), 1106a (2), MSHR305 (1), MSHR668 (1), 1026b (1), MSHR5854 (1); Gp. 4, MSHR668 (1), 406e (1), 1106a (6), K96243 (1), 1026b (3), MSHR5855 (1), MSHR5848 (1). The significant levels compared to that in naïve cells are shown: †, P < 0.05; ¶, P < 0.01; §, P < 0.001; ‡, P ≤ 0.0001