Dysregulation of TNF-α and IFN-γ expression is a common host immune response in a chronically infected mouse model of melioidosis when comparing multiple human strains of Burkholderia pseudomallei

Table 2 Types of spleens isolated from BALB/c mice after aerosol exposure to clinical isolates of B. pseudomallei (Bp)

				No. Spleens Recovered in each Category^a,b
	Bp Strain	LD₅₀ (CFU)^c	No. survivors left in exposure group (s) considered ^d	Normal appearing Gp 2	Enlarged, Swollen Gp 3	Chronic, Infected, pyogranulomatous lesion Gp 4
1	BpMSHR5855	0.35	9/20	7	0	1
2	BpHBPUB10134a	0.99	11/20	8	0	0^e
3	Bp406e	2.79	6/20	3	1	2
4	Bp1026b	4.10	8/10	0	1	3
5	BpMSHR668	4.42	7/20	5	1	1
6	BpMSHR5848	4.66	13/20	6	1	1
7	BpMSHR5858	4.97	5/10	0	0	4
8	Bp22^f	5.00	11/20	5	0	2
9	BpMSHR305	5.98	6/10	0	1	3
10	BpK96243	25.1	13/20	3	1	2
11	Bp1106a	4266	10/20	0	2	8^g
	Total:			37	8	27

^a Naïve, unexposed BALB/c mice (n = 10) are in Group 1
^b Spleens were recovered 22–70 days post-infection
^c LD₅₀ from [29]
^d Number of survivors from one exposure group (initially 10 mice) or two exposure groups (initially 20 mice) from where we obtained mice to evaluate after 21 days
^e No infected survivors were recovered because they died before day 21
^f LD₅₀ was determined late and not reported in [29]
^g This group includes 2 infected spleens that were used for immunohistochemical analysis

ISSN: 1471-2172