Table 5 Characteristics of spleens/sera in BALB/c mice after aerosol exposure to strains of B. pseudomallei
General Properties | Category of Spleens from Aerosol Exposed Mice | |||
|---|---|---|---|---|
Naïve | Normal appearing | Enlarged | Chronically Infected | |
Gp 1a | Gp 2 | Gp 3 | Gp 4 | |
Weight, size | ~ 100 mg | ~ 100 mg | > 100 mg - 400+ mg | > 400 mg - > 1500 mg |
Pyogranulomatous (visable) lesion | No | No | No | Yes |
CFU recovered | No | No | 0–240 | 103 - 109 |
Elevated IgG response | No | No, or very low | Yes | Yes |
Increased inflammatory cells | No | Very littleb | Yesc | Yesc |
Increased cytokine/ chemokine expression | ||||
Serum | No | No | No | Yes |
Spleen extract | No | Yesd | Yes | Yes |
Ex vivo expression cytokines | No | Very littlee | Yes | Yes f |
Intracellular cytokine expression | ||||
TNF-α | No | Very little | Yes | Yes |
IFN-γ | No | No | Yes | Yes |