Table 2 Candidate reference gene primers and details
Symbol | Primer and Probe Sequences (5′-3′) | Exons | Average Efficiency (%)a |
|---|---|---|---|
ACTB | F: ACAGAGCCTCGCCTTTG R: CCTTGCACATGCCGGAG P: /5HEX/TCATCCATG/ZEN/GTGAGCTGGCGG/3IABkFQ/ | 1–2 | 99.5 |
GAPDH | F: ACATCGCTCAGACACCATG R: TGTAGTTGAGGTCAATGAAGGG P: /5HEX/AAGGTCGGA/ZEN/GTCAACGGATTTGGTC/3AIBkFQ/ | 2–3 | 96.8 |
RPL13a | F: GCCGCCCCTGTTTCAAG R: CTCGACCATCAAGCACCAG P: /5HEX/AGAAACCCT/ZEN/GCGACAAAACCTCCT/3IABkFQ/ | 1b-3 | 99.3 |
RPS18 | F: GTTCCAGCATATTTTGCGAGT R: GTCAATGTCTGCTTTCCTCAAC P: /5HEX/TCTTCGGCC/ZEN/CACACCCTTAATGG/3IABkFQ/ | 2–3 | 99.5 |
SDHA | F: TTTGATGCAGTGGTGGTAGG R: CAGAGCAGCATTGATTCCTC P: /5HEX/TGCAACAGT/ZEN/GTGTGACCTGGTAGG/3IABkFQ/ | 3–4 | 99.5 |
UBE2D2 | F: GTACTCTTGTCCATCTGTTCTCTG R: CCATTCCCGAGCTATTCTGTT P: /5HEX/CCGAGCAAT/ZEN/CTCAGGCACTAAAGGA/3IABkFQ/ | 6–8 | 99.3 |