Skip to main content

Table 3 Reference gene reliability analysis on unstimulated and live influenza A virus stimulated PBMCs using Silver’s method, geNorm, BestKeeper and NormFinder

From: Reliable reference genes for the quantification of mRNA in human T-cells and PBMCs stimulated with live influenza virus

  ACTBGAPDHRPL13aRPS18SDHAUBE2D2
MethodMeasurePBMCT-cellPBMCT-cellPBMCT-cellPBMCT-cellPBMCT-cellPBMCT-cell
SilverσCq0.520.5621.2041.290.8030.8210.5380.5680.5640.6010.4930.541
geNormM1.2810.3620.8250.8540.5560.3120.520.2780.530.3110.5370.287
BestKeeperSD0.290.200.840.960.520.430.250.330.260.290.220.35
r0.7370.5040.5870.6880.227−0.1730.7380.8730.4010.4680.8360.907
NormFinder (Stability Value)Intragroup (all samples)0.0670.1000.8100.8730.4790.4960.0680.0740.1920.2190.0730.074
Intergroup (± virus)0.0940.1470.5720.5080.4360.4020.0740.080.1520.1730.0460.099
Intergroup (Y/O)0.0340.0390.260.2810.1540.1610.0440.0290.0580.0660.0220.023
  1. Expression stability of candidate reference genes was calculated from n = 10 paired unstimulated and influenza A/Victoria/375 stimulated PBMCs and T-cells