Reliable reference genes for the quantification of mRNA in human T-cells and PBMCs stimulated with live influenza virus

Table 3 Reference gene reliability analysis on unstimulated and live influenza A virus stimulated PBMCs using Silver’s method, geNorm, BestKeeper and NormFinder

		ACTB		GAPDH		RPL13a		RPS18		SDHA		UBE2D2
Method	Measure	PBMC	T-cell	PBMC	T-cell	PBMC	T-cell	PBMC	T-cell	PBMC	T-cell	PBMC	T-cell
Silver	σCq	0.52	0.562	1.204	1.29	0.803	0.821	0.538	0.568	0.564	0.601	0.493	0.541
geNorm	M	1.281	0.362	0.825	0.854	0.556	0.312	0.52	0.278	0.53	0.311	0.537	0.287
BestKeeper	SD	0.29	0.20	0.84	0.96	0.52	0.43	0.25	0.33	0.26	0.29	0.22	0.35
BestKeeper	r	0.737	0.504	0.587	0.688	0.227	−0.173	0.738	0.873	0.401	0.468	0.836	0.907
NormFinder (Stability Value)	Intragroup (all samples)	0.067	0.100	0.810	0.873	0.479	0.496	0.068	0.074	0.192	0.219	0.073	0.074
	Intergroup (± virus)	0.094	0.147	0.572	0.508	0.436	0.402	0.074	0.08	0.152	0.173	0.046	0.099
	Intergroup (Y/O)	0.034	0.039	0.26	0.281	0.154	0.161	0.044	0.029	0.058	0.066	0.022	0.023

Expression stability of candidate reference genes was calculated from n = 10 paired unstimulated and influenza A/Victoria/375 stimulated PBMCs and T-cells

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