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Fig. 4 | BMC Immunology

Fig. 4

From: The DNA methyltransferase inhibitor, guadecitabine, targets tumor-induced myelopoiesis and recovers T cell activity to slow tumor growth in combination with adoptive immunotherapy in a mouse model of breast cancer

Fig. 4

Guadecitabine boosts the CD8+ T cell anti-tumor response in the spleen. IFNγ production by a dLN lymphocytes and b splenocytes from tumor-bearing mice following ex vivo restimulation for 3 h with ionomycin and PMA. c Total number of IFNγ-producing CD8+ splenocytes. d Representative immunoflourescent staining of frozen tumor sections for anti-CD8α (green) and anti-IFNγ (blue). e Quantitative RT-PCR for Ifng message on whole tumor. Relative quatification normalized to housekeeping gene Hrpt. Significance determined by unpaired student’s T-test for a-c. Significance determined by Mann-Whitney non-parametric analysis for E. error bars represent SD. ns = not significant; *:p value< 0.0332; ****:p value< 0.00001

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BMC Immunology

ISSN: 1471-2172

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